Title:

The use of Giant Miscanthus (Miscanthus × Giganteus) in 2G bioethanol production

Alternative title:

Wykorzystanie Miskanta Olbrzymiego (Miscanthus × Giganteus) w produkcji bioetanolu 2G

Creator:

Smuga-Kogut, Małgorzata ; Bugajski, Sławomir ; Piskier, Tomasz ; Walendzik, Bartosz

Subject:

giant miscanthus ; bioethanol ; chemical treatment of biomass ; enzymatic hydrolysis ; alcoholic fermentation

Description:

The aim of the study was to conduct the process of bioethanol obtaining from biomass using chemical treatment and enzymatic hydrolysis. Different concentrations of sodium hydroxide (5 and 10%) were used for the delignification process, and enzymatic hydrolysis was carried out using three commercial cellulolytic preparations (Cellic® CTec2, cellulase from Trichoderma reesei and cellulase from Aspergillus species). The final step involved an alcoholic fermentation process using Saccharomyces cerevisiae TYPE II yeast. After enzymatic hydrolysis, the content of reducing sugars was determined in the samples, and the fermentation yield was controlled by determining the ethanol content by pycnometry. The use of chemical pretreatment increased the yield of the whole process by at least 50%. The content of reducing sugars after hydrolysis depended on the type of enzyme preparation used for hydrolysis and the use of NaOH in pretreatment. The highest content of reducing sugars (45.8 g/dm3) was achieved in a sample of material that was purified with 5% NaOH, and enzymatic hydrolysis was carried out using Cellic® CTec2. This means an efficiency of the enzymatic hydrolysis process equal to 94.69%. The concentration of bioethanol after alcoholic fermentation in this sample was 0.509 g/l.

Place of publishing:

Koszalin

Publisher:

Politechnika Koszalińska

Date:

2023

Type:

artykuł w czasopiśmie

Language:

eng

Is part of:

Rocznik Ochrona Środowiska. Vol. 25, s. 413-422

Rights:

Biblioteka Politechniki Koszalińskiej

Access rights:

internet

License:

Creative Commons BY-SA 4.0